Journal: Cell reports

Article Title: Astrocytes Amplify Neuronal Dendritic Volume Transmission Stimulated by Norepinephrine

doi: 10.1016/j.celrep.2019.11.092

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: rabbit anti GFAP , Phosphosolution , Cat# 620-GFAP; RRID:AB_2492124.

Techniques: Virus, Plasmid Preparation, Recombinant, Software

Journal: Journal of neuropathology and experimental neurology

Article Title: Hereditary ferritinopathy: a novel mutation, its cellular pathology, and pathogenetic insights.

doi: 10.1093/jnen/64.4.280

Figure Lengend Snippet: FIGURE 9. Hyaline deposits. Pallidum. (A) Pleomorphic hyaline deposits, some containing nuclei, within the same area of GPe as in Figure 6A. (B) Prussian-blue reactivity. (C) PTAH reactivity. (D) Polyclonal anti-ferritin immunoreactivity. (E) Rare association of hyaline deposits with GFAP-immunoreactive astrocyte. (F) HO-2-immunoreactivity. Original magnifications: (A) Hematoxylin and eosin, 1503; (B) Perl’s stain, 2203; (C) 2203; (D) 2503; (E) 2203; (F) 2503.

Article Snippet: In order to identify the cell(s) of origin of the unique vacuolated nuclei, antibodies to S-100 (polyclonal, 1:20k, Lot #941; Innovation Foundation, Toronto, Ontario, Canada), GFAP, CD68 (monoclonal, 1:200, Lot #120201; DAKO), and carbonic anhydrase II (CAII) (polyclonal, 100-401-136/4383, 1:15k with retrieval; Rockland, Gilbertsville, PA) were applied to sections of basal ganglia and cerebellum, while antibodies to synaptophysin (monoclonal, 1:100 with retrieval, #M0363-UC/0403; Biogenex, San Ramon, CA), PGP 9.5 (polyclonal, 1:1,000 with retrieval, Lot #11830; Biogenesis, Handsown, NH), neurofilament protein 2F11 (monoclonal, 1:350, #M0762/117 DAKO) (101), and amyloid precursor protein (APP A4; monoclonal, 1:20K with heat retrieval, #23080547, Chemicon) were applied to the section of basal ganglia.

Techniques: Staining

Journal: Nature Communications

Article Title: Neuropeptide SP protects against colitis and linked anxiety-like behavior through the putative roles of gut microbiota and metabolite inositol

doi: 10.1038/s41467-025-67904-0

Figure Lengend Snippet: a Diagram illustrating the mouse model of colitis employed in this study, and inositol treatments are indicated (Some schematic elements were created by Figdraw.com). b Daily body weight changes. n = 8 mice/group. c Diseases activity index. n = 6 mice/group. d Macroscopic pictures of colons. e H&E-stained colon sections. Scale bar = 100 μm. n = 5 independent experiments. f Immunofluorescence staining for E-cadherin (red)/DAPI (blue) in colon tissues. Scale bar = 100 μm. n = 4 independent experiments. g The mRNA level of inflammatory cytokines ( IL-1β , IL-6 and TNF-α ) in the colon. n = 4 mice/group. h , i Representative movement tracks in the open field test and related bar graphs (Distance traveled, distance of center region and speed). n = 6 mice/group. j , k Track plot of the elevated plus maze, and statistical analysis including percentage of time spent in the open arms and percentage of times entering the open arms. n = 6 mice/group. l Representative H&E-stained hippocampus sections of three groups. Scale bar = 100 μm. n = 6 independent experiments. m Photomicrographs of Nissl staining in the hippocampus. Scale bar = 100 μm. n = 6 independent experiments. n The mRNA expressions of downstream cytokines ( IL-6 , IL-1β , TNF-α and IL-10 ) in the hippocampus tissue. n = 5 mice/group. o Representative immunohistochemistry images of Iba-1 in hippocampus, Scale bar = 100 μm. n = 3 independent experiments. p The concentration of GABA in hippocampus tissue. n = 6 mice/group. q The mRNA expression of GABA A Rα1 in hippocampus. n = 5 mice/group. r The protein level of GABA B R detected by western blot. n = 3 independent experiments. s Double immunofluorescence staining for GFAP (red)/GAT1 (green) in the DG and CA1 regions of hippocampus tissue. Scale bar = 50 μm. n = 3 independent experiments. Data were presented as means ± SD. For body weight change, two-way repeated-measures ANOVA was performed and the rest of the statistics was analyzed using one-way ANOVA followed by Tukey’s multiple comparisons test. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001. Source data are provided as a file.

Article Snippet: Briefly, after deparaffinization, rehydration, and antigen retrieval, slides were blocked with donkey serum for 30 min at room temperature following incubation with primary antibodies against GFAP (1:1000, A8335, 1:200, NB100-53809, USA), Iba-1 (DF6442, 1:200, Affinity, China), E-cadherin (PB9561, 1:200, Boster, China), Mucin 2 (bsm-60016R, 1:100, Bioss, China), GABA A Rα1 (1:200, 12708-1-AP, Proteintech, China), calcium/calmodulin-dependent protein kinase II (CaMKII) (1:50, ab76703, Abcam, UK) and p-IKBα (1:100, WLH3930, Wanleibio, China) at 4 C overnight.

Techniques: Activity Assay, Staining, Immunofluorescence, Immunohistochemistry, Concentration Assay, Expressing, Western Blot, Double Immunofluorescence Staining

Journal: Precision Clinical Medicine

Article Title: Drug pair-derived synergistic therapy of flavonoids luteolin and astragaloside IV promotes neural repair following spinal cord injury via antioxidant and neuroprotective effects

doi: 10.1093/pcmedi/pbaf037

Figure Lengend Snippet: Lut-AST promoted regrowth of NF-positive neural fibers across the glial scar and 5-HT expression following SCI. (A) Double-labeled immunofluorescence images of NF (green) and GFAP (red) in longitudinal sections surrounding the injury site of rats in the Lut-AST treatment group and SCI group at 5 weeks post-injury, with corresponding quantitative analysis shown in (B) and (C). (D) Representative images showing 5-HT (green) staining in spinal cord longitudinal sections around the injury lesion of Lut-AST-treated rats and SCI rats at 5 weeks after SCI. Data are shown as mean ± SEM ( n = 6), * P < 0.05, ** P < 0.01, *** P < 0.001, one-way ANOVA followed by Tukey’s post hoc test.

Article Snippet: The sections were then stained with primary antibodies including: NF (Cell Signaling Technology, USA), glial fibrillary acidic protein (GFAP; Boster, China), ionized calcium-binding adaptor molecule 1 antibody (Iba1; WAKO, Japan), and 5-HT (WAKO, Japan), and incubated overnight at 4°C.

Techniques: Expressing, Labeling, Immunofluorescence, Staining